OUR ACCUSCRIPT? REVERSE TRANSCRIPTASE (RT)
DELIVERS THE BEST REVERSE TRANSCRIPTION ACCURACY
DURING FIRST-STRAND CDNA SYNTHESIS. IT IS THE
IDEAL CHOICE FOR ANY APPLICATION THAT REQUIRES
PREMIUM ERROR-FREE cDNA.
Reverse transcriptases exhibit significantly higher error
rates than other known DNA polymerases, introducing
errors at frequencies of one per 1,500 to 30,000
nucleotides during cDNA synthesis1 and are the major
contributor of errors in reverse transcriptase PCR
(RT-PCR), particularly when using ultra high-fidelity PCR
enzymes (Table 1). These RT-introduced errors are then
amplified exponentially during the PCR step and as a
result, you must spend a significant amount of time and
effort identifying and correcting errors after RT-PCR.?
To solve the problem of low reverse transcription accuracy,
we developed AccuScript? High Fidelity Reverse
Transcriptase*, which is engineered to deliver the highest
transcription accuracy possible while promoting full-length
cDNA synthesis, high yield and excellent RT-PCR
performance.